Hematopoietic stem cell–engrafted NOD/SCID/IL2R null mice develop human lymphoid systems and induce long-lasting HIV-1 infection with specific humoral immune responses
نویسندگان
چکیده
1Department of Virology, Division of Medical Science, Medical Research Institute, Tokyo Medical and Dental University, Japan; 2Department of Molecular Virology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan; 3AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; 4Department of Infectious Diseases, National Research Institute for Child Health and Development, Tokyo, Japan; 5Central Institute for Experimental Animals, Kanagawa, Japan; and 6Department of Pediatrics and Developmental Biology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan
منابع مشابه
Hematopoietic stem cell-engrafted NOD/SCID/IL2Rgamma null mice develop human lymphoid systems and induce long-lasting HIV-1 infection with specific humoral immune responses.
Critical to the development of an effective HIV/AIDS model is the production of an animal model that reproduces long-lasting active replication of HIV-1 followed by elicitation of virus-specific immune responses. In this study, we constructed humanized nonobese diabetic/severe combined immunodeficiency (NOD/SCID)/interleukin-2 receptor gamma-chain knockout (IL2Rgamma(null)) (hNOG) mice by trans...
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1. Notta F, Doulatov S, Dick JE. Engraftment of human hematopoietic stem cells is more efficient in female NOD/SCID/IL-2Rgc-null recipients. Blood. 2010; 115(18):3704-3707. 2. Shultz LD, Lyons BL, Burzenski LM, et al. Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells. J Immunol. 2005;174(10):6477-6489. 3. Agli...
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Here we report that a new nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse line harboring a complete null mutation of the common cytokine receptor chain (NOD/SCID/interleukin 2 receptor [IL2r] null) efficiently supports development of functional human hemato-lymphopoiesis. Purified human (h) CD34 or hCD34 hCD38 cord blood (CB) cells were transplanted into NOD/SCID/IL2r null ne...
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Immunodeficient mice engrafted with human HSCs support multidisciplinary translational experimentation, including the study of human hematopoiesis. Heightened levels of human HSC engraftment are observed in immunodeficient mice expressing mutations in the IL2receptor common chain (IL2rg) gene, including NOD-scid IL2r null (NSG) mice. Engraftment of human HSC requires preconditioning of immunode...
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